Capabilities:
– High-speed multi-color image acquisition with spinning disk
– High resolution multi-color image with 6 laser lines (405, 440, 488, 514, 561 and 640 nm)
– Fast and high-resolution image acquisition with EMCCD camera (30.6 frame/s, 1000 x 1000 pixels)
Cost:
Academic users: $40/h, Industry users: $60/h
Specifications:
Microscope:
Leica DMi8 inverted microscope
Prior motorized stage
Yokogawa CSU-X1 spinning disk scan head
Illumination and Detection:
Solid-laser lines for 405, 440, 488, 514, 561 and 640 nm
Available for DAPI, CFP, GFP, Alexa488, FITC, YFP, RFP, mCherry, Propidium iodide in switchable filter wheel
Alexa405/DAPI 435-485
CFP 460-500
YFP 525-555
Alexa 488/GFP/FITC 507-543
RFP/PI 570-620
Alexa 635/Cy5 612-688
Objectives:
10x/NA 0.3/Air (WD 11.13mm)
20x/NA 0.7/Glycerol (WD 2.0mm)
40x/NA 1.25/Oil (WD 0.1mm)
63x/NA 1.3/Glycerol (WD 0.28mm)
100x/NA 1.4 /Oil (WD 0.09mm)
Camera:
Hamamatsu 9100-02 CCD camera (30.6 frame/s, 1000 x 1000 pixels) for all objectives
Hamamatsu 9100-23B CCD camera (70.4 frame/s, 512 x 512 pixels) for 100x/Oil objective
Computer:
Improvision Volocity software operated by Windows 7